Answer :
when you added primary antibody to the wells, If the antigen was not detected, the main antibody was washed off.
Antibodies and proteins shield you from harmful chemicals when they come into touch with your body. Antibodies are created by your immune system to bind to these foreign chemicals and remove them from your body. Immunoglobulin is another term for an antibody. A foreign substance that enters your body is referred to as an antigen. This includes venom, allergens, bacteria, viruses, fungus, and other poisons. Your immune system makes an antibody protein that it employs to find and eliminate particular antigen. Surface molecules on antigens are different from ones your body typically produces. Your immune system thus recognizes an antigen as soon as it enters your body. Your immune system requests antibody defense in order to fight against this antigen invasion. The B cell produces antibodies (specialized white blood cells). A B cell expands and clones when it comes into touch with an antigen. These plasma cells, also known as cloned B cells, continuously produce antibodies into your lymphatic and blood systems.
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