Answer :

The correct option is template from 3' to 5' direction and primer from 5' to 3' direction.

The template strand is read from 3' to 5' direction. This is due to the reason that RNA primase that synthesized short strands of RNA from the template works only in one direction, which 5' to 3'. Owing to this, primer strand will be 5' to 3' and template will be from 3' to 5'.

DNA synthesis is bidirectional due to antiparallel nature of DNA. The opposite strands lead to synthesis in their respective 5' to 3' direction. DNA primase, helicase, topoisomerase, ligase and DNA polymerase are significant enzymes responsible for DNA replication.

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