A scientist determined the rate of an enzyme-catalyzed reaction by measuring the amount of product formed over time. The following curve was generated from the data collected. Use the graph shown to answer questions 5-6. 5. The rate of the reaction could also be determined by: A. measuring the change in the amount of enzyme B. measuring the change in the amount of substrate C. measuring the change in salt concentration D. adding more substrate E. adding more enzyme 6. What is the most likely explanation for the change in the slope of the line between 3 and 5 minutes? A. The enzyme had denatured. B. The enzyme had achieved its maximum velocity at the point of saturation. C. Alarge amount of the substrate had been consumed. D. An allosteric in hibitor appeared. E. There was a dramatic change in the pH