If you were to use a mutated plasmid in the pGlo experiment, one that no longer had a functional beta-lactamase gene, which statement below is the most likely observable outcome? Bacteria will not be able to grow on ampicillin plates Bacteria will not be able to break down arabinose The bacteria will not be able to express GFP There will be a significant change in the bacterial colony appearance The plasmid will no longer be able to be transformed into the bacterial cells



Answer :

Bacteria are microbes with a cell structure simpler than that of many other organisms. Their control centre, containing the genetic information, is contained in a single loop of DNA. Some bacteria have an extra circle of genetic material called a plasmid rather than a nucleus.

A competent strain of bacteria is injected with the DNA (often in the form of a plasmid) during transformation so that the bacteria can reproduce the desired sequence in numbers adequate for additional research and/or modification. A recombinant plasmid that has been inserted into a bacterial cell causes the cell to start expressing the genes on it. By employing the bacterial cell's machinery, DNA polymerase finds the ori- the origin of replication- and begins to duplicate the plasmid. A fundamental molecular biology approach is the heat shock method of transforming plasmid DNA into E. coli. It involves putting an alien plasmid or ligation product inside of bacteria.

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