Answer :
The DNA sequence is 3' T C G T T C A G T 5' and the mRNA sequence will be 5' AG C A A G U C A 3'.
There are a number of widely used methods to detect and determine the abundance of specific mRNAs in total RNA or poly RNA samples. Here, we review four common methods: Northern blot analysis, nuclease protection assay in situ hybridization, and reverse transcription-polymerase chain reaction.
During transcription, the DNA of a gene serves as a template for complementary base-pairing, and an enzyme called RNA polymerase II catalyzes the formation of pre-mRNA molecules, which are then processed into mature mRNA. RNA polymerase reads the code in the 3'-5' direction from the template strand and produces the mRNA strand in the 5'-3' direction.
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