Materials Provided in Lab Kit: Water soluble pen (vis-à-vis) Pre-measured yeast in a plastic bag 1 package sucrose (table sugar) Metric ruler 3 - 15-ml conical tubes 1 plastic cup (holder for 15-ml conical tubes) 3 water balloons Plastic pipette Provided by Student: Room-temperature water (20 to 25°C) Warm tap water (41 to 46°C) Spoon Adhesive tape Small funnel or make a Paper funnel: Cut a piece of notebook paper in half. Taking one of the far corners and roll it into the center so that the paper's edge is touching the middle of the paper. With your other hand, bring the other corner and roll it over top so that it wraps around the other corner. Adjust to fit in end of tube. Tape in place (See the finished funnel in Figure 2.) When you have completed the lab, make sure to take a picture of the indicated results. Then discard the balloons and solutions following the clean-up directions below. Wash the rest of the equipment for future use. Do not discard. Procedure: Step One: Prepare tubes: 1. Use the water-soluble marker to label three 15 ml tubes: C (Control); E1 (Experimental #1); & E2 (Experimental #2). 2. Use your metric ruler to measure 1.5 cm from the bottom of each tube. Draw the line with the water-soluble marker. (Figure 1). Figure 4: Measure 1.5cm from the bottom of 15 ml conical tube 3. Draw an additional line on tubes E1 and E2 at 2 cm. 4 | P a g e 4. Using the funnel, CAREFULLY add 1.5 cm of yeast to all 3 tubes. (Figure 2) 5. Using the funnel, CAREFULLY add 0.5 cm (second line) of sucrose (sugar packet) to tubes E1 and E2. Figure 5: Add 1.5 cm of yeast using paper funnel 6. Use the plastic pipette to add enough room temperature (20 to 25°C) tap water to tubes C and E1 to fill to the 6-ml line on the tube. Add caps and shake to thoroughly mix the contents. 7. Use the plastic pipette to add enough warm (41 to 46°C) tap water to the 6-ml line on tube E2. Add cap and shake to thoroughly mix the contents. 8. Remove caps from tubes. Secure the tubes (have a friend or family member hold the tube or secure