Which of the following experimental strategies could be used to block the IP3 branch but not the DAG branch of the signaling pathway that involves phosphatidylinositol-4,5-bisphosphate?
Which of the following experimental strategies could be used to block the IP3 branch but not the DAG branch of the signaling pathway that involves phosphatidylinositol-4,5-bisphosphate?
addition of a cAMP phosphodiesterase inhibitor
blocking the GPCR involved
inhibition of protein kinase A
inhibition of phospholipase C
adding an antagonist of the IP3 receptor

Question

05-12-2022
Biology

Answered

Which of the following experimental strategies could be used to block the IP3 branch but not the DAG branch of the signaling pathway that involves phosphatidylinositol-4,5-bisphosphate?
Which of the following experimental strategies could be used to block the IP3 branch but not the DAG branch of the signaling pathway that involves phosphatidylinositol-4,5-bisphosphate?
addition of a cAMP phosphodiesterase inhibitor
blocking the GPCR involved
inhibition of protein kinase A
inhibition of phospholipase C
adding an antagonist of the IP3 receptor

Answer :

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Qwclear Qwclear · Answer 1 · 2022-12-07T09:55:09-05:00

The experimental strategy which could be used to block the IP3 branch but not the DAG branch of signaling pathway is by adding an antagonist of the IP3 receptor, which suggests that option E is the right answer.

Agonist-occupied G protein- coupled receptors (GPCRs) promote exchange of GTP for GDP on Gα-subunits. IP3 is an inositol phosphate signaling molecule, which is made by hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), which is a phospholipid that is located in the plasma membrane, by phospholipase C (PLC). IP3, DAG, and Ca2+ are second messengers in the phosphoinositol pathway. Diacylglycerol (DAG) is a secondary lipid messenger for transducing the signals downstream of many receptors which are expressed by hematopoietic cells.

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