Trendymwah8531 Trendymwah8531

08-11-2022
Biology

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Amylase is a protein that catalyzes the conversion of starch to simple sugars. Amylase activity in an aqueous solution can be measured by using iodine as a starch indicator. A solution containing iodine and starch will have a dark-blue color, whereas a solution containing iodine but no starch will have a light-brown color. The color change of an iodine solution from dark blue to light brown can be used to measure the rate at which starch is converted to simple sugars.

Answer :

RupaBera RupaBera

12-11-2022

Measuring the emergence of the product or the removal of the reactants allows for the detection of enzyme catalysis.

How is the amylase activity of starch measured?

We'll use the rate at which the substrate (starch) is broken down to calculate the activity of amylase in saliva samples. Iodine and starch easily combine to create a purple tint. To measure the purple color's intensity quantitatively, we shall utilize a spectrophotometer.

How can you check if a solution contains amylase?

Iodine does not react with glucose but instead produces a blue to black complex with starch. Iodine's red or yellow tint is the sole color that appears when it is added to a glucose solution. Consequently, the quicker starch loses its blue hue, The amylase enzyme is operating more quickly.

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