Answer :
Outside the mitochondrial matrix, when hydrogen ions are being pumped, to the inner mitochondrial membrane, as well as the gap occurs between both inner and outer membranes, the result is Proton gradient creation.
Protons are moved from the matrix and released into the intermembrane space when electrons move along the chain of electron transport. As a result, the proton motive force—also known as an electric potential or proton gradient—is produced across the inner membrane. Only through certain channels may protons that are overabundant in the intermembrane space cross the inner membrane and return to the matrix along their gradient of concentration.
Therefore, we may infer that the production of a proton-motive force occurs as a result of the pumping of hydrogen ions from the mitochondrial matrix over the inner membrane and into the intermembrane gap.
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