what powers the action of helicase at the replication fork, where it opens up the double helix? choose one: a. dna hydrolysis b. pyrophosphate hydrolysis c. the attachment of single-strand dna-binding proteins to the lagging-strand template d. atp hydrolysis e. dna synthesis

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20-12-2022
Biology

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what powers the action of helicase at the replication fork, where it opens up the double helix? choose one: a. dna hydrolysis b. pyrophosphate hydrolysis c. the attachment of single-strand dna-binding proteins to the lagging-strand template d. atp hydrolysis e. dna synthesis

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jaspreetsharma jaspreetsharma · Answer 1 · 2022-12-25T03:23:01-05:00

Helicase opens the double helix at the origin of replication with the help of DNA hydrolysis.

Helicases move and unwind DNA using the energy from nucleoside triphosphate (NTP) hydrolysis, producing the single-stranded template (ssDNA) needed for correct DNA polymerase replication. Because they split double-stranded DNA to single strands, allowing every strand to be duplicated, DNA helicases are crucial for DNA replication. DNA restriction enzymes unwind DNA at locations known as origins during DNA replication, where synthesis will begin. The bidirectionally expanded DNA replication forks are opened by the helicase. Around the replication fork, single-strand binding proteins cover the DNA to stop it from winding back on itself. To stop supercoiling, topoisomerase binds to the area before the replication fork.

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