Answer :
Option D: overlapping fragments of genomic DNA is generally the starting material for whole-genome shotgun sequencing of genomic DNA
To determine the DNA sequence of an organism's genome, scientists use a technique called shotgun sequencing. The technique entails randomly dividing the genome into tiny DNA fragments that are then individually sequenced. A computer programme searches for DNA sequence overlaps and uses them to put the genome's parts back together in the right order.
Genomic DNA overlapping segments serve as the raw material for whole-genome shotgun sequencing.
In a lab, shotgun sequencing can be used to determine the DNA sequence of a genome.
The procedure involves randomly slicing the genome into tiny DNA fragments, which are then each individually sequenced.
A computer program scans the DNA sequences for overlaps, and then uses those overlaps to reassemble the fragments in the correct order to rebuild the genome.
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