denaturation: is irreversible. is caused by the disruption of the noncovalent interactions. is the result of protein insertion into a membrane. is caused by disruption of covalent bonds. is the unfolding of a protein.



Answer :

Proteins become denatured when their noncovalent connections are broken and they unfold. Therefore, statements 2 and 5 are correct.

Proteins become denatured when their secondary and tertiary structures are changed, rendering them unable to carry out their intended functions. Some proteins can regain their former shape after being denatured. Therefore, denaturation can often be reversible. The denaturation is only strong enough to break the weak non-covalent bonds. But they are not strong enough to break covalent bonds like peptide bonds. So denaturation only alters the secondary and other high-order structures of proteins. This way the denaturation process helps to unfold the protein structure. Some denaturing agents of proteins are heat, alcohol, detergent, pH changes, and heavy metal salts.

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