30.which of the following is not an application of polymerase chain reaction? a.production of many copies of an unknown piece of dna b.making copies of a foreign gene for use in making recombinant dna c.dna fingerprinting of a crime suspect d.linking two dna molecules together

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19-12-2022
Biology

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30.which of the following is not an application of polymerase chain reaction? a.production of many copies of an unknown piece of dna b.making copies of a foreign gene for use in making recombinant dna c.dna fingerprinting of a crime suspect d.linking two dna molecules together

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contexto1220 contexto1220 · Answer 1 · 2022-12-23T00:45:20-05:00

The making copies of a foreign gene for use in making recombinant dna is incorrect.

PCR usually pro- duces double-stranded merchandise. For some of programs the double- stranded PCR product need to be transformed to the Single-stranded form. Such appli- cations consist of the sequencing of PCR merchandise or their use as hybridization probes.The PCR system will increase and reduces the temperature of the pattern in automatic, programmed steps. Initially, the combination is heated to denature, or separate, the double-stranded DNA template into unmarried strands.

The combination is then cooled in order that the primers anneal, or bind, to the DNA templateThe new fragments of DNA which are made at some point of PCR additionally function templates to which the DNA polymerase enzyme can connect and begin making DNA. The end result is a massive variety of copies of the unique DNA phase produced in a enormously quick length of time.