which of the following sequences of events in the production of recombinant dna are in the correct order? a.cut dna with restriction enzymes, mix dna with plasmid dna, add dna ligase b.mix dna with plasmid dna, cut with restriction enzymes, add dna ligase c.add dna ligase, cut dna with restriction enzymes, mix dna with plasmid dna d.mix dna with plasmid dna, add dna ligase, cut with restriction enzymes

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16-12-2022
Biology

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which of the following sequences of events in the production of recombinant dna are in the correct order? a.cut dna with restriction enzymes, mix dna with plasmid dna, add dna ligase b.mix dna with plasmid dna, cut with restriction enzymes, add dna ligase c.add dna ligase, cut dna with restriction enzymes, mix dna with plasmid dna d.mix dna with plasmid dna, add dna ligase, cut with restriction enzymes

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qwmoon qwmoon · Answer 1 · 2022-12-20T08:40:19-05:00

The correct sequence of events in the production of recombinant DNA is cut insert DNA and plasmid DNA with restriction enzymes, mix insert DNA with plasmid DNA, add DNA ligase.

Enzymes and a variety of laboratory procedures are used in recombinant DNA technology to modify and isolate specific DNA sequences. Using this technique, one can join (or splice) DNA from several species or produce genes with novel functions. Recombinant DNA is the name given to the resultant copies.

The process of fusing two or more DNA molecules to produce a hybrid is known as recombinant DNA. Restrictions endonucleases and ligases are two classes of enzymes that enable the technique. When a restriction endonuclease detects a particular DNA sequence, it makes cuts inside or near that sequence.

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