Answer :
The ability to read a DNA template from 3' to 5' is required for DNA replication, transcription, and translation.
According to the Central Dogma of Molecular Biology, DNA creates RNA, which creates proteins. The process of copying DNA to RNA is known as transcription, and the process of using RNA to make proteins is known as translation.
The opening of the double helix and separation of the DNA strands, priming of the template strand, and assembly of the new DNA segment are the three major steps in replication.
DNA replication necessitates the action of three major enzymes, each with a distinct role: Helicase is responsible for unzipping and unwinding the DNA molecule. DNA polymerase adds a new complementary strand of DNA to each of the original helicase-separated halves.
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