luluh1328 luluh1328

12-12-2022
Mathematics

Answered

describe a procedure that you would use to localize the protein dynein in tissue sections of esophagus and trachea. indicate the results that you would expect from such an analysis.

Answer :

Tutorconsortium423 Tutorconsortium423

18-12-2022

Therefore, the five steps are taken to localize the protein dynein in tissue sections of the esophagus and trachea.

What is localization?

Any procedure that involves moving, attaching to, or otherwise maintaining a cell, substance, or cellular entity in one place, like an organelle or protein complex. Substance localisation can also be accomplished through selective degradation.

In this case,

the steps that are done in order to localize the protein dynein in tissue sections of the esophagus and trachea are as follows:

1. Histoclear removes the medium to enable the labeling of the antibodies

2. to rehydrate the sample, add water.

3. Using BSA blocking solution after Triton X- 100, which ruptures membranes and makes antigens more accessible to antibodies

(4) Add the dynein antibody.

5. include a secondary antibody that is likewise peroxidase-active (adds specificity)

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