7) Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?
I-The primers hybridize to the target DNA.
II-The mixture is heated to a high temperature to denature the double stranded target DNA.
III-Fresh DNA polymerase is added.
IV-DNA polymerase extends the primers to make a copy of the target DNA.
A) II, I, IV.
B) I, III, II, IV.
C) III, IV, I, II.
D) III, IV, II.
E) II, III, IV.

Question

09-12-2022
Biology

Answered

7) Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?
I-The primers hybridize to the target DNA.
II-The mixture is heated to a high temperature to denature the double stranded target DNA.
III-Fresh DNA polymerase is added.
IV-DNA polymerase extends the primers to make a copy of the target DNA.
A) II, I, IV.
B) I, III, II, IV.
C) III, IV, I, II.
D) III, IV, II.
E) II, III, IV.

Answer :

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kjuli1766 kjuli1766 · Answer 1 · 2022-12-17T08:19:42-05:00

Option A. B) I, III, II, IV. best describes the complete sequence of steps occurring during every cycle of PCR.

Polymerase chain reaction (abbreviated PCR) is a laboratory method for swiftly producing (amplifying) millions to billions of copies of a particular section of DNA, that could then be studied in greater element.

PCR─denaturation, annealing, and extension─as shown inside the first cycle, and the exponential amplification of goal DNA with repeated cycling.Polymerase Chain reaction. PCR or Polymerase Chain response is a way utilized in molecular biology to create numerous copies of a sure DNA phase. This method changed into developed in 1983 by Kary Mullis, an American biochemist. PCR has made it possible to generate tens of millions of copies of a small phase of DNA.

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