after dideoxy dna sequencing, you find the sequence of your unknown dna sample (template in the reaction) by: a. reading the results on the gel directly from top to bottom (5' -> 3') b. reading the results on the gel directly from bottom to top (5' -> 3') c. taking the reverse complement of the sequence on the gel d. none of the options listed here

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05-12-2022
Biology

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after dideoxy dna sequencing, you find the sequence of your unknown dna sample (template in the reaction) by: a. reading the results on the gel directly from top to bottom (5' -> 3') b. reading the results on the gel directly from bottom to top (5' -> 3') c. taking the reverse complement of the sequence on the gel d. none of the options listed here

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fahiskt9087 fahiskt9087 · Answer 1 · 2022-12-08T02:43:45-05:00

Correct answer is option a.After dideoxy DNAsequencing, the sequence of your unknown dna sample (template in the reaction) byreading the results on the gel directly from top to bottom (5' -> 3').

DNA sequencing refers to general laboratory techniques for determining the exact sequence of nucleotides or bases in a DNA molecule. Sequences of bases (often referred to by the first letter of their chemical names: A, T, C, and G) encode biological information that cells use for growth and operation.WebDNA sequence shown on the electropherogram of the readout gone i.e. generated by the laser scanner. This method is known as the dideoxy chain termination method. The sequencing method is based on the use of chain terminators, dideoxynucleotides (ddNTPs).

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