The best evidence that life evolved from prokaryotic ancestors is that the oldest fossils found are prokaryotes.
There are thousands of distinct kinds of organisms that have been preserved in fossil records, indicating that the earliest forms of life on Earth were unicellular. The multicellular organisms that make up the majority of life on Earth gradually developed as cells began to function differently as they remained together.
The oldest fossils of bacteria were found about 3.5 billion years ago, while the oldest eukaryotic organisms were found about 2.1 billion years ago, which is almost 1.5 billion years younger than the oldest fossils.
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