Answer :
There is a binding site for a protein called the catabolite activator protein upstream of the promoter (CAP).
What is mechanism of binding of CAP protein ?
The binding of the CAP protein bends the DNA and facilitates RNA polymerase attachment, which dramatically improves transcription of the lac operon.
- With the help of bound CAP, RNA polymerase may bind to the lac operon promoter. If lactose is available, the operator will release the lac repressor (by binding of allolactose).
- This makes it possible for RNA polymerase to continue transcription of the operon on the DNA. Catabolite activator protein is required for RNA polymerase to start transcription of the lac operon should attach to CAMP.
- The lactose (lac) operon promoter is positively regulated by the catabolite gene activator-cyclic AMP complex (CAP), which binds to DNA 61.5 bp upstream of the transcription start point.
Learn more about CAP protein here:
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