An organism's capacity to use the enzyme nitrate reductase to convert nitrate (NO3) to nitrite (NO2) is assessed using nitrate broth.
Additionally, it assesses how well organisms can convert nitrate and nitrite into molecular nitrogen through the process of nitrification.In tryptic soy broth medium, nitrate is the preferred terminal electron acceptor for the anaerobic growth of K27. Nitrite functions as a terminal electron acceptor and a toxic reagent: low levels of nitrite promote K27's anaerobic growth, while high levels of nitrite prevent it.Because it is a component of all the various amino acids used to make proteins, the chemical element nitrogen is essential to all living things. Nitrates disperse in water before being ingested by plants. Animals must consume plants or other animals to obtain the amino acids needed for protein synthesis.
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