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when a vector that employs the lac z gene as a second marker is used in a cloning experiment, bacteria that harbor the recombinant dna will give rise to a. red colonies. b. blue colonies. c. all of the choices are correct. d. white colonies. 0.2 points



Answer :

Bacteria that harbor the recombinant DNA will give rise to white colonies. Thus, option D is correct.

Bacteria develop on strong media as colonies. A colony is characterized as an obvious mass of microorganisms all beginning from a single mother cell, in this manner a colony constitutes a clone of microbes all hereditarily alike.

In the recognizable proof of microbes and organisms, much weight is put on how the life form develops in or on media. This workout will assist you to distinguish the social characteristics of a bacterium on an agar plate - called colony morphology.

Plasmids that are commonly utilized in quality cloning are around 3000 nucleotide sets in length, have an ampicillin resistance quality, and have a quality that encodes the protein beta-galactosidase.

The quality encoding this chemical is called the LacZ or blue color quality. The beta-galactosidase chemical will utilize galactose sugar as a substrate.

In case an uncommon form of this sugar called X-gal is put within the media, a flawless thing happens interior the microbes that have the blue color quality.

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