Chromosomes align in the cell equator occurs at the metaphase stage.
Mitosis is the process of cell division into two daughter cells with identical DNA base pairs. Mitosis takes place in four stages namely prophase, metaphase, anaphase, and telophase.
In the second stage of mitosis, namely the metaphase stage when the nuclear membrane is gone, so the chromosomes come out of the nucleus and are aligned in the the equator cell. Chromosomes are in the densest state so that they are shorter and thicker and the chromatids repel each other, each attached to the opposite end of the fiber.
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