Answer :
Heating the mouth of the check tube creates an air contemporary that enables prevents the airborne microorganisms from falling into the tube even as the tube's cap is off. If airborne bacteria do fall into the tube, they will contaminate the medium.
This guarantees that no microorganisms enter the mouth of the vessel to infect the lifestyle or the medium. Passing the mouth of the bottle via a flame produces a convection current far from the hole, and helps to prevent infection.
This guarantees that no microorganisms input the mouth of the vessel to contaminate the subculture or the medium. Passing the mouth of the bottle via a flame produces a convection present-day far away from the hole, and facilitates to save you contamination.
Warm the mouth of the open test tube by passing it thru the flame of the Bunsen burner. Heating creates convection currents, which deliver airborne debris far from the mouth of the tube, stopping contamination of the culture or medium within.
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