ATP SYNTHASE enzyme located in the membrane of mitochondria synthesizes the most atp during the overall process of cellular respiration.
Adenosine diphosphate (ADP) and inorganic phosphate are used to create the energy storage molecule adenosine triphosphate (ATP) by a protein called ATP synthase (Pi). Because it creates a P-O connection to modify ADP, it is referred to as a ligase (phosphodiester bond). ATP synthase is a molecular machine. Energy-wise, it is preferable for the process to proceed the other way; producing ATP from ADP and Pi is undesirable. This process is accelerated by coupling ATP synthesis during cellular respiration to a proton (H+) concentration gradient across the inner mitochondrial membrane in eukaryotes or the plasma membrane in bacteria.
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