Answer :
Because of a mutation in Lac O (the operator), the repressor protein I may not bind in this mutant.
What would happen if the lac operon's Z gene was altered?
Because lactose is the only energy source present in the medium, coli cells with a mutation in the lac operon's Z gene are unable to develop there. Lactose is unable to enter cells from the media.
What circumstances cause the lacZ gene to be expressed?
IIt only manifests when both lactose and glucose are lacking. Two regulators that control the operon's "on" and "off" states in response to lactose and glucose concentrations are lac repressor and catabolite activator protein (CAP).
What does lacZ mutation mean?
The inability to manufacture the ß-galactosidase gene product is the lac Z gene's phenotype in a cell. Without this enzyme, cells are unable to digest lactose, which prevents them from growing if lactose is the only source of energy.
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