The addition of short microtubules to unpolymerized GTP-tubulin would have the biggest impact on the lag phase of microtubule polymerization in vitro.
These findings imply that GTP functions in microtubule assembly by first giving tubulin a conformation that is conducive to polymerization, and then by hydrolyzing GTP bound to tubulin to irreversibly change the equilibrium in favor of polymerization.
What mitotic stage are microtubules polymerizing in?
Prophase is the time when spindle microtubules begin to polymerize from tubulin units.
When relatively small molecules, known as monomers, chemically interact to form a very large chainlike or network molecule, known as a polymer, the process is known as polymerization. The monomer's constituent molecules may all be identical or may represent two, three, or more distinct entities.
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