The purpose of using restriction endonucleases is to cut DNA at specific location to obtain desired gene and to clone this gene to produce sticky end.
What is restriction endonuclease and how is it used to produce sticky ends?
- Restriction endonuclease is a restriction enzyme used to cut DNA at specific point and obtain the desired product.
- Restriction endonuclease are of different types , the first restriction endonuclease was named Hind-III.
- We always use same kind of restriction endonuclease to cut the cloning vector with a purpose of producing same kind of sticky ends.
- Then after the sticky ends are joined using DNA ligase to produce and finally obtain the whole product.
- The most frequent use of restriction endonuclease is in the first step of DNA replication process.
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