What is the purpose of using restriction endonucleases to cut dna to produce sticky ends?.

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04-11-2022
Biology

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What is the purpose of using restriction endonucleases to cut dna to produce sticky ends?.

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qwoffwhite qwoffwhite · Answer 1 · 2022-11-14T10:14:27-05:00

The purpose of using restriction endonucleases to cut DNA to produce sticky ends is due to the way they keep two sections of DNA together so that DNA ligase can join them, sticky ends are useful in the process of cloning.

So-called sticky ends are those produced by enzymes that leave single-stranded overhangs. Restriction endonucleases primarily serve as a form of defense against foreign genetic material, particularly against DNA from bacteriophages. Recombination and transposition are the additional activities ascribed to these enzymes.

Double-stranded DNA is halved by restriction enzymes. Depending on the restriction enzyme, a cut will produce a sticky or blunt end. Sticky ends are more helpful for molecular cloning because they make sure the human DNA fragment is inserted into the plasmid in the correct direction. After being isolated from bacterial sources, it is either cut at or close to the recognition site.

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