Answer :

Amino acid and sodium hydroxide used to make a buffer solution.

What is buffer solution?

buffer solution is a waterproof solvent based solution in  which consists of a mixture of  containing a weak acid and  the conjugate base of the weak acid, or a weak base of the solution and the conjugate acid of the weak base. They resist to a  change in pH upon to the  dilution or upon the addition of small amounts of a  acid/alkali to them. Buffer solutions resist of a  change in pH when smallest amounts of a stronger acid or a stronger base are added.

Sol-acitic acid is a weaker acid and  its conjugate base is the acetate anion. That's why the addition of the  strong base, hydroxide, which neutralized half of a  acetic acid created a buffer and  solution because we are having  significant amounts of both acetic acid and its conjugate base, the acetate anion, in the solution.

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