As we know CRISPR are repetitive sequences in bacterial DNA, that work as a "self vaccine" that is to say contain the genetic material of the viruses that have attacked them, this way they recognize the alien element and cut their DNA. Taking this into consideration we can say that the correct answer is, What sequence of nucleotides does the guide RNA need to include, to kill the bacterium? as is necessary a guide where to start the process, a primer.