A drug that inhibits dna ligase but not dna polymerase is added to a cell. Explain how dna replication would be affected as a result of the drug.

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27-10-2022
Biology

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A drug that inhibits dna ligase but not dna polymerase is added to a cell. Explain how dna replication would be affected as a result of the drug.

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qwrose qwrose · Answer 1 · 2022-10-31T13:52:29-04:00

DNA replication would be affected in this way, the backbone of the new DNA would be missing because the new DNA fragments would not fuse together.

DNA deterioration can be due to the surroundings UV light, as an example, forms cyclobutene dimers in DNA between thymidine residues that halt the replication machinery development. DNA harm is likewise by and large as a result of pollution.

Considering that hydroxyurea (HU) and methyl methane sulfonate (MMS) are fork-arresting retailers, researchers frequently employ them in DNA experiments. Template DNA is alkylated by way of MMS, but deoxyribonucleotides (dNTP) are depleted by using HU, which stops DNA synthesis and causes fork stalling.

Reactive oxygen species, which might be produced in the course of regular mobile metabolism, also can damage DNA and prevent DNA from replicating. The DNA structure itself should function yet some other bodily barrier. complex three-dimensional systems like telomeres, centromeres, and ribosomal DNA are formed via repeated DNA sequences, which can be challenging to replicate.

Learn more about DNA replication here:

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