The enzyme DNA ligase is likely to come from a species that is able to live within acid mine drainage.
By facilitating the formation of a phosphodiester bond, the enzyme known as DNA ligase makes it easier to link DNA strands together. Living creatures use it to repair single-strand breaks in duplex DNA, although some forms may also repair double-strand breaks (i.e. a break in both complementary strands of DNA).
In order to fully repair the DNA after a single-strand break, DNA ligase constructs the last phosphodiester bond using the complementary strand of the double helix as a template.
Both DNA replication and DNA repair need DNA ligase (see Mammalian ligases). In gene cloning, purified DNA ligase is used to link DNA molecules to create recombinant DNA.
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