The rules which govern the construction of plasmids from the three kinds of restriction fragments are an even number of fragments must be present in plasmids made up of those with one BamHI and one HindIII.
One of the three types of restriction fragments in recombinant plasmids must therefore be present twice. A plasmid is a tiny, circular DNA molecule that exists independently of the host cell's genome.
The use of restriction enzymes is necessary for the construction of plasmids. At specified locations known as restriction sites, these enzymes are utilized to cut the DNA. A different enzyme called DNA ligase is subsequently used to combine the resultant fragments.
Currently, restriction enzymes are used to digest certain DNA sequences, and DNA ligase is then used to join the resultant DNA fragments together to create plasmids.
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