Answer :
The bands ended up in those relative positions this might be caused by the gel's uneven pore size.
As a result, we should see less blue migration than purple movement. We have, however, obtained the opposite outcome.
This might happen if the gel is cast using partially melted agar. As a result, the middle lane's pore size is larger, which improves the speed of the blue dye loaded in the well relative to the purple dye-filled well.
Always moving in the direction of the oppositely charged pole are molecules. The wells can be positioned in the middle of the gel if the charges of the molecules to be sorted are unknown, allowing the molecules to move freely through the gel in the direction of either pole.
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