Light microscope experiment
1. Put a thin sample of tissue (e.g onion epidermis) onto a microscope slide.
2. Add a few drops of suitable stain/ dye (e.g iodine)
3. Place a coverslip on top of the tissue and place slide under microscope stage.
4. Use objective lens with lowest magnification and focus on sample.
5. Increase magnification and focus to see different features of cell.



Answer :

Utilizing a microscope

  1. Set the stage, the flat ledge that the slide rests on, to the lowest setting.
  2. The glass slide should be placed on the stage. Push it beneath the clips carefully to prevent the cover slide from moving or breaking.
  3. Pick the objective lens with the smallest power.
  4. Once you can see the cells, carefully turn the coarse focus slider.
  5. Once the cells are in focus and are clearly visible, carefully turn the fine focus knob.
  6. To see the cells in greater detail, repeat steps 1 through 5 with the higher power magnification.

Magnification calculations

The overall magnification needed to see an object is calculated by multiplying the eyepiece and objective lens magnifications.

Total magnification is the sum of the magnifications of the eyepiece and objective lenses.

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