If you mutated the laci gene resulting in a mutant lac repressor protein that could bind to the lac operator but not to allolactose, what would be the consequence for transcription of the lac operon?.

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25-10-2022
Biology

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If you mutated the laci gene resulting in a mutant lac repressor protein that could bind to the lac operator but not to allolactose, what would be the consequence for transcription of the lac operon?.

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GouravDayma GouravDayma · Answer 1 · 2022-10-28T07:27:15-04:00

A lactose sensor is the lac repressor. When lactose is present, it ceases to function as a repressor, blocking transcription of the operon in its usual state.

What is allolactose produced by?

As stated in this article, allolactose, a derivative of lactose, is produced by -galactosidase as a byproduct in the cleavage of lactose to glucose and galactose, and it acts as an inducer in vivo. In addition to converting lactose to allolactose (A), the enzyme -galactosidase also breaks down allolactose into glucose and galactose.

What causes allolactose to behave?

The lac repressor is bound by allolactose, which changes its shape and prevents it from binding DNA. An inducer, or tiny chemical that prompts the expression of a gene or operon, includes allolactose.

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