Compared with pure cultures grown in the lab, microbes in nature usually experience a wider range of environmental conditions, more variation in conditions over time, and more contact with other organisms. Therefore, the same organism in nature will typically grow slower than in pure culture.
By freeze drying, many microorganisms may be efficiently maintained. Cells can be efficiently maintained by freezing them in a solution containing a lyoprotectant (often sucrose) and vacuum-separating the water (sublimation).
To better understand and treat caused illnesses, a pure bacterial culture is still necessary for the research of its virulence, sensitivity to antibiotics, and genomic sequencing.
Because it makes it easier to apply recombinant DNA technology by enabling the separation of clones, the isolation of bacteria in pure culture is crucial.
The observation of the specimen in issue requires the development of pure culture procedures.
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