a new restriction endonuclease is isolated from a bacterium. this enzyme cuts dna into fragments that average 4,096 base pairs long. like many other known restriction enzymes, the new one recognizes a sequence in dna that has twofold rotational symmetry. from the information given, how many base pairs of dna constitute the recognition sequence for the new enzyme?

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25-10-2022
Biology

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a new restriction endonuclease is isolated from a bacterium. this enzyme cuts dna into fragments that average 4,096 base pairs long. like many other known restriction enzymes, the new one recognizes a sequence in dna that has twofold rotational symmetry. from the information given, how many base pairs of dna constitute the recognition sequence for the new enzyme?

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contexto1019 contexto1019 · Answer 1 · 2022-11-04T09:04:36-04:00

For the identification of cleavage or cutting capacity of the particular newly isolated restriction endonuclease enzyme, we can provide the entire known nucleotide sequence during restriction activity, after that we can do sequencing for the analysis of at which specific position DNA is cutting down by that particular restriction enzyme.

Sequencing is the method used to know the genomics size of long as well as short stretch of DNA results in a shorter time period. There are two types of sequencing, first is ancient sequencing techniques such as Sanger sequencing and second one is next generation sequencing. Nowadays the next generation sequencing method is widely used for sequencing. These next generation sequencing is totally based on computer based technique.

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