Answer :
Some microbiologists prefer to open and close quickly the culture tube instead of flaming its mouth prior and after transferring bacteria to or from it because it prevents spills more effectively because it eliminates the necessity of an open flame
What are the risks associated with utilising direct burning to control bacteria?
Contamination danger is increased. Not all contagious components will be promptly deactivated and destroyed when an item is exposed to open flames.
Flaming the Test Tube's Mouth: A convection current is produced when the mouth of a tube is passed past the flame of a Bunsen burner, forcing air out of the tube. By doing this, pollutants from the air are kept out of the tube.
Flaming is used to warm the bottle's opening and produce air convection currents that move away from the opening rather than sterilising it (i.e., updraft). The heated, rising air helps keep contaminants and other impurities out of the bottle.
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