most vectors have a second genetic marker that is made non-functional if an insert is in the multiple-cloning site. this disruption of the marker's function is called

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18-10-2022
Biology

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most vectors have a second genetic marker that is made non-functional if an insert is in the multiple-cloning site. this disruption of the marker's function is called

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ArunVenu252 ArunVenu252 · Answer 1 · 2022-11-01T11:16:52-04:00

Recombinant DNA refers to a vector that contains foreign DNA. Cosmids, viral vectors, plasmids, and synthetic chromosomes are the four main categories of vectors.

What two objects are typically employed in genetic engineering as vectors?

Plasmids and bacteriophages are the two most used kinds of vectors. Plasmids are circular DNA molecules with one or more genes that are present inside the bacterial cell independently. The host bacteria depends on these genes in a significant way.

The bacterial colonies with the recombinant plasmids and colonies without them are mixed together while copying a gene using plasmid vectors. All the colonies have the same appearance, but some have adopted the plasmids containing the human gene, while others have not.

These were the original gene cloning vectors. These are present in eukaryotes, archaea, and bacteria. These DNA molecules are self-replicating, extrachromosomal, natural molecules.

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