Answer :
Dilution is needed to bring the unknown’s absorbance within the range of the standard curve.
If the dilution procedure is documented, it is possible to calculate the full-strength concentration after determining the diluted unknown quantity. Beer’s Law only applies to diluted solutions; hence it is preferable to dilute the unknown than to create new standards.
WHAT PURPOSE DOES A SPECTROPHOTOMETER SERVE?
Spectrophotometers gauge transmittance (T) and absorbance (A) (T). Photons per second are used to quantify the light’s intensity. A blank sample, which does not absorb light when light passes through it, is represented as (I).
What is the spectrophotometer’s fundamental tenet?
The principle of the spectrometer. The spectrophotometer is a device that calculates how much light a substance absorbs.
The spectrophotometer measures the light intensity of a sample by passing a light beam through it.
How does a spectrophotometer function and what is it?
A spectrophotometer is a device that counts the number of photons (light’s energy) absorbed after they have passed through a sample solution.
The spectrophotometer can also be used to determine the concentrations (amounts) of known chemical substances.
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