Addition of a phosphate group to a protein increases the protein's potential energy. Which statements about addition of phosphate are likely accurate? Select all that apply.
Responses

The increased potential energy after phosphate addition can be used by the protein to do work in the cell.
The increased potential energy after phosphate addition can be used by the protein to do work in the cell.

The phosphate group makes hydrophobic interactions with neighboring R-groups.
The phosphate group makes hydrophobic interactions with neighboring R-groups.

The negative charges in phosphate can repel neighboring R-groups, eventually changing the shape of the protein.
The negative charges in phosphate can repel neighboring R-groups, eventually changing the shape of the protein.

Addition of phosphate is a spontaneous process that does not require coupling with other reactions.
Addition of phosphate is a spontaneous process that does not require coupling with other reactions.