To isolate Neurospora (haploid) auxotrophs from prototrophs, you perform a large-scale mutagenesis using a reagent that induces base substitutions. You learned in class that you should culture them on the complete medium first to collect all possible axuotrophs and prototrophs and then test them on the minimal media plate (M&M plate) by making replicas. But there is another way to discover many, not all, auxotrophs using the minimal media plates ONLY. Dr. T laid out a basic plan below. He left out culture conditions of Neurospora blank for this experiment. Complete the plan by filling in the boxes labeled with "Culture condition" and indicate which ones are auxotrophs you look for by using dots wisely in two circles labeled with "M&M plates as you learned in class. Note that the Neurospora's normal culture condition is 30°C. This means that auxotrophs you aim to isolate would die on M&M plates at 30°C. However, you must let them survive on your master plate before identifying auxotrophs.
You ARE NOT allowed to add any amino acid supplements to minimal media plates. In other words, you are allowed to use ONLY minimal media plates in this question. Think about environmental conditions other than amino acid supplements.​