Given the following 100bp DNA sequence (this is one strand from a double stranded region of
DNA written from 5' to 3') that you would like to amplify using PCR.
AATTATTATT CGGGTTATTC ATGAAGCTTG GTGGATCCAT TTCCTTGGGC
GGATCCGCAA GCTTGGGATC CCAGGCGGTA TCTGAAACAA TTTGGTTAAA
The amplified sequence was incubated with the restriction enzyme HindIII that recognizes the
sequence AAGCTT and cuts between the two As (see image below)
5...AAGCTT...3
3... TTCGAA...5
7.1 How many fragments would be produced from this digest?
7.2 Give the exact sizes of the bands