1. The following results were obtained for an enzyme-catalysed reaction
Assignment
Substrate concentration (mmol I'): 5.0 Initial velocity (pmol I' min): 147
Calculate Km and Vmax
2. For the following enzyme-catalyzed reaction whose results are displayed below, determine the Vmax and the Km of the enzyme
[S] (ummol/L) 2
V (umol/L/min) 5
10
20
20
40
40
64
60
80