1. The following results were obtained for an enzyme-catalysed reaction

Assignment

Substrate concentration (mmol I'): 5.0 Initial velocity (pmol I' min): 147

Calculate Km and Vmax

2. For the following enzyme-catalyzed reaction whose results are displayed below, determine the Vmax and the Km of the enzyme

[S] (ummol/L) 2

V (umol/L/min) 5

10

20

20

40

40

64

60

80