You are plating 16 wells of a 24 well plate with A673 cells. You want 4 treatment groups: Untreated, 5μM THC-V, 25μM THC-V, and 50μM THC-V. You have 4 wells of each treatment. You will put 2 mL of the treatment in each well. What total
volume of media and THC-V will you need to make the 50μM treatment of THC-V? Assume a stock concentration of 10mM THC-V. Remember to calculate for one
additional well. Show your work.
Volume of media:
Volume of THC-V: