Shown here is a restriction map of a 12-kb linear plasmid isolated from cells of Borrelia burgdorferi, a spirochete bacterium transmitted by the bite of Ixodes ticks that causes Lyme disease. The symbols D, P, C, H, K, S, and A represent cleavage sites for the restriction enzymes DdeI, PstI, ClaI, HindIII, KpnI, SacI, and AluI, respectively. In the accompany- ing gel diagram, show the positions at which bands would be found after digestion of the plasmid with the indicated restriction enzyme or enzymes.



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