The students prepared stock solutions of compound 1 in four different concentrations in ph 7 buffer. a stock solution of the enzyme was prepared by diluting 0.100 ml of the commercial preparation to 25.0 ml in the buffer solution. experiments were initiated by mixing 1.0 ml of each substrate solution with 1.0 ml of the enzyme solution. the initial rates vo were measured for each trial. the students then plotted 1/vo versus 1/[s] (figure 1) to determine km, vmax, and [e]t for the four trials.