Calculations on Stock Solutions be sure to include the amount of water added and include all units Calculate how to use the 20% stock solution of SDS to make a100 ml of a 0.1% SDS working solution 7. Calculate how to use the 0.5 M Tris stock solutions to make the 350 ml ofa 10mM Tris working solution. Calculations on Multiple Stock Solutions be sure to include the amount of water added and include all units 8. Calculate how to use the M Tris and 0.2 M EDTA stock solutions to make the 400 ml of a of 10 mM Tris and mM EDTA solution 9,Calculate how t0 use 3 M NaCl and 10% NP4O stock solutions to make 500 ml of & 300 mM NaCl and 0.2% NP4O working solution: Calculations on Simple Dilutions be sure to include the amount ofwater added and include all units 10, Calculate the volumes necessary to make 200 ml of a 1.5 dilution of a sample: 11, Calculate the volume of cell suspension containing 800,000 cells/ml You would need to obtain total of 250,000 cells Practice Calculations On Serial Dilutions-be sure to include the amount of water added and include all units 12 Calculate and describe (or diagram) how You would use a 10% SDS solution to prepare final volume of 20 ml of each of the following solutions: 8%, 4%, 2%k, 1% 0.5% 13. Calculate and, describe (or diagram) how You would use 4 5 M Tris solution to prepare 200 ml of each of the following solutions: IM, 1Q0 mM; 10 mM TmM,0.1 mM: How much of the first dilution should YOu make to perform the following dilution? 14,100 ml of 4 I:lO dilution